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William A. Mohler, Ph.D.

Mohler Lab

 
 
PUBLICATIONS
Plotnikov SV, Millard AC, Campagnola PJ, and Mohler WA.   Characterization of the myosin-based source for second-harmonic generation from muscle sarcomeres. Biophys J. 2006 90(2):693-703.

del Campo JJ, Staber CJ, Reenan RA, and Mohler WA.  (2005)  Fusing partner cells require mutual expression of the autonomous cellular fusogen EFF-1.  Current Biology.  Submitted. 

Plotnikov SV, Juneja V, Isaacson AB, Mohler WA, and Campagonla PJ*.  Optical clearing for improved contrast in second-harmonic generation imaging of skeletal muscle. Biophys J. 2006 90(1):328-39.

Mohler WA.  (2005) Cell-cell fusion: transient channels leading to plasma membrane merger. In: Cell-Cell Channels, Frantisek Baluska ed., Landes Bioscience, Georgetown, TX. Submitted. 

Balla S, Thapar V, Luong T, Faghri T, Huang C-H, Rajasekaran S, del Campo JJ, Shinn JH, Moher WA, Maciejewski MW,  Gryk MR, Piccirillo B, Schiller SR, and Schiller MR. Minimotif miner, a new tool for investigating protein function.  Nature Methods, 2006 3(3):175-177. 

Wang J, Mohler WA,  Savage-Dunn C. (2005) C-terminal mutants of C. elegans Smads reveal tissue-specific requirements for protein activation by TGF-beta signaling.  Development 132(15): 3505-3513

del Campo JJ, Opoku-Serebuoh E., Isaacson AB, Scranton VL, Tucker M., Han M., and Mohler WA.  (2005) Fusogenic activity of EFF-1 is regulated via dynamic localization in fusing somatic cells of C. elegans.  Current Biology 15(5): 413-423. 

Mohler WA and Isaacson AB.  (2005) Imaging Embryonic Development in Caenorhabditis elegans. In: Imaging in Neuroscience and Development, Rafael Yuste and Arthur Konnerth eds., Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY. 

Arur S, Uche UE, Rezaul K, Fong M, Scranton V, Cowan AE, Mohler W, and Han DK.  (2003)  A novel engulfment ligand from apoptotic cells identified by quantitative proteomics.  Developmental Cell. 4(4): 587-598.

Millard AC, Campagnola PJ, Mohler W, Lewis A, and Loew LM.  (2003)  Second harmonic generation imaging microscopy.  Methods Enzymol.  361: 47-69.

Mohler W, Millard AC, and Campagnola PJ.  (2003)  Second harmonic generation imaging of endogenous structural proteins.  Methods  29(1): 97-109.

Campagnola P and Mohler WA.  (2003) Nonlinear optical spectroscopy and imaging of structural proteins in living tissues.  Optics and Photonics News.  14(6): 40-45.

Eliceiri KW, Rueden C, Mohler WA, Hibbard WL, and White JG.  (2002)  Analysis of multidimensional biological image data.  Biotechniques  33(6):1268-73

Mohler WA, Shemer G, del Campo JJ, Valansi C, Opoku-Serebuoh, Scranton V, Assaf N, White JG, and Podbilewicz B. 2002. The type I membrane protein EFF-1 is essential for developmental cell fusion. Dev. Cell. 2: 355-362.

Campagnola PJ, Millard AC, Terasaki M, Hoppe PE, Malone CJ, and Mohler WA . 2002. Three-dimensional high-resolution second-harmonic imaging of endogenous protein structural proteins in biological tissues. Biophys. J. 82: 493-508.

Campagnola PJ, Clark HA, Mohler WA , Lewis A, and Loew LM. 2001. Second-harmonic imaging microscopy of living cells. J. Biomed. Opt. 6: 277-286.

Skop AR, Bergmann D, Mohler WA , and White JG. 2001. Completion of cytokinesis in C. elegans requires a brefeldin A-sensitive membrane accumulation at the cleavage furrow apex. Curr. Biol. 11: 735-746.

Heid PJ, Raich WB, Smith R, Mohler WA , Simokat K, Gendreau SB, Rothman JH, and Hardin J. 2001. The zinc finger protein DIE-1 is required for late events during epithelial cell rearrangement in C. elegans. Dev. Biol. 236: 165-180.

Hoier EF, Mohler WA , Kim SK, Hajnal A. 2000. The Caenorhabditis elegans APC-related gene apr-1 is required for epithelial cell migration and Hox gene expression. Genes Dev, 14:874-886.

Mohler WA and Squirrell JM. 2000. Multiphoton imaging of embryonic development. In: Imaging Neurons: A Laboratory Manual. Yuste R, Lanni F, Konnerth A. eds. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY.

Charlton CA, Mohler WA , Blau HM. 2000. Neural cell adhesion molecule (NCAM) and myoblast fusion. Dev Biol, 221:112-119.

Mohler WA. 1999. Visual reality: using computer reconstruction and animation to magnify the microscopist's perception. Mol Biol Cell, 10:3061-3065.

Mohler WA , Simske JS, Williams-Masson EM, Hardin JD, and White JG. 1998. Dynamics and ultrastructure of developmental cell fusions in the Caenorhabditis elegans hypodermis. Curr Biol, 8:1087-1090.

Mohler WA and White JG. 1998. Multiphoton laser scanning microscopy for four-dimensional analysis of Caenorhabditis elegans embryonic development. Optics Express, 3:325-331.

Mohler WA and White JG. 1998. Stereo-4-D reconstruction and animation from living fluorescent specimens. Biotechniques, 24:1006-1012.

Charlton CA, Mohler WA , Radice GL, Hynes RO, and Blau HM. 1997. Fusion competence of myoblast rendered genetically null for N-cadherin in culture. J Cell Biol, 138:331-336.

Yang JT, Rando TR, Mohler WA , Rayburn H, Blau HM, and Hynes RO. 1996. Genetic analysis of alpha 4 integrin functions in the development of mouse skeletal muscle. J Cell Biol, 135:829-835.

Mohler WA and Blau HM. 1996. Gene expression and cell fusion analyzed by lacZ complementation in mammalian cells. Proc. Nat. Acad. Sci., USA 93:12432-12427.

 
LAB MEMBERS
Name Position E-mail
William Mohler Principal Investigator wmohler@neuron.uchc.edu
Dan Vasilescu Research Associate vasilescu.uchc.edu
Victoria Scranton Research Assistant vscranton@nso2.uchc.edu
Sergey Plotnikov Postdoctoral Fellow splotnikov@neuron.uchc.edu
Star Dunham-Ems Postdoctoral Fellow sdunham@uchc.edu
John O'Donnell Graduate Student odonnell@student.uchc.edu
Jessica Shinn Graduate Student jshinn@student.uchc.edu
Alumni: Jacob del Campo, Kristy Freeman, Ariel Isaacson, Eugene Opoku-Serebuoh, Jianjun Wang, Beata Zubrowski

 

 
ROTATIONS

Mohler Lab Research Projects
Formation and structure of giant cells. Live imaging of cells, tissues, and development.

Cell fusion in the
nematode C. elegans:

Cell fusion in
mammalian development:

Tissue imaging in
disease and aging:

Gene expression in
C. elegans development:

Function of EFF-1 in cell membrane fusion.
-Analysis of EFF-1 domain structure and function.
-New proteins involved in worm cell fusion

Proteins involved in myogenic cell fusion.
-siRNA screen of candidate genes.
-Chemical compound screen for modulators of cell fusion.

Second harmonic generation (SHG) from muscle.
-Structural source of SHG from sarcomeres.
-Quantitative SHG-image analysis.

Multi-dimensional movies of fluorescent embryos.
-3D time-lapse imaging via confocal microscopy.

Regulation of EFF-1 expression in development.
-Tissue-specific regulation of the eff-1 promoter.
-Stochasticity of gene activation.

Development of fusing cells in the mouse.
-in vivo imaging of cell fusion in embryos.
-in vitro assays of fusion in non-muscle cell-types.

SHG imaging of non-muscle tissues.
-Collagen imaging in skin and bone.

Bionformatics of image data.
-Software for processing and correlation of embryo movies.

Cellular dynamics of cell fusion.
-Localization and control of fusion machinery.
-Fusion pore size and structure.
-Cytoskeletal dynamics during fusion.

 

*Click link to see a relevant publication for each project