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Bruce J. Mayer, Ph.D.

Mayer Lab

 
PUBLICATIONS
Sallee NA, Rivera GM, Deuber JE,, Vasilescu D, Mullins RD, Mayer BJ, Lim WA. The pathogen protein EspFU hijacks N-WASP-mediated actin polymerization using mimicry and multivalency. Nature 2008; 454:1005-1008.

Antoku S, Saksela K, Rivera GM and Mayer BJ. A crucial role in cell spreading for the interaction of Abl PxxP motifs with Crk and Nck adaptors. J Cell Sci 2008; 121:3071-3082.

Sharma A and Mayer BJ. Phosphorylation of p130Cas initiates Rac activation and membrane ruffling. BMC Cell Biology 2008, 9:50.

Mayer BJ. Clues to the evolution of complex signaling machinery. Proc Natl Acad Sci USA 2008, 105:9453-9454.


Machida K, Thompson CM, Dierck K, Jablonowski K, Kärkkäinen S, Liu B, Zhang H, Nash PD, Newman DK, Nollau P, Pawson T, Renkema GH, Saksela K, Schiller MR, Shin DG, Mayer BJ*. High throughput phosphotyrosine profiling using SH2 domains. Mol. Cell; 26: 899-915.

Rivera GM, Antoku S, Gelkop S, Shin SY, Hanks SK, Pawson T, Mayer BJ. Requirement of Nck adaptors for actin dynamics and cell migration stimulated by platelet-derived growth factor B. Proc Natl Acad Sci USA 2006; 103:9536-9541.

Dierck K, Machida K, Voigt A, Thimm J, Horstmann M, Fiedler W, Mayer BJ, Nollau P. Quantitative multiplexed profiling of cellular signaling networks using phosphotyrosine-specific DNA-tagged SH2 domains. Nat Meth 2006; 3:737-744.

Rivera GM, Briceño CA, Takeshima F, Snapper SB, Mayer BJ. Inducible clustering of membrane-targeted SH3 domains of the adaptor protein Nck triggers localized actin polymerization.  Curr Biol 2004; 14:11-22.

Sharma A, Antoku S, Fujiwara K, Mayer BJ. 2003. Functional Interaction Trap: A strategy for validating the functional consequences of tyrosine phosphorylation of specific substrates in vivo.  Mol Cell Proteomics 2:1217-1224.

Machida K, Mayer BJ, Nollau P. 2003. Profiling the global tyrosine phosphorylation state. Mol Cell Proteomics 2:215-233.

Smith JM and Mayer BJ.  2002. Abl:  Mechanisms of regulation and activation.  Front Biosci 7:d31-42.

Fujiwara K, Poikonen K, Aleman L, Valtavaara M, Saksela K, Mayer BJ. 2002. A single-chain antibody / epitope system for functional analysis of protein-protein interactions.  Biochemistry 41:12729-12738.

Parrini MC, Lei M, Harrison SC, Mayer BJ. 2002. Pak1 kinase homodimers are autoinhibited in trans and dissociated upon activation by Cdc42 and Rac1. Mol Cell 9: 73-83.

Nollau P, Mayer BJ. 2001. Profiling the global tyrosine phosphorylation state by Src Homology 2 domain binding. Proc Natl Acad Sci USA 98: 13531-13536.

Miyoshi-Akiyama T, Aleman LM, Smith JM, Adler CE, Mayer BJ. 2001. Regulation of Cbl phosphorylation by the Abl tyrosine kinase and the Nck SH2/SH3 adaptor. Oncogene 20:4058-4069.

Rohatgi R, Nollau P, Kirschner MW, Mayer BJ. 2001. Nck and phosphatidylinositol 4,5-bisphosphate synergistically activate actin polymerization through the N-WASP-ARP2/3 pathway. J Biol Chem 276:26448-26452.

Mayer BJ. 2001. SH3 domains: complexity in moderation. J Cell Sci 114:1253-1263.

Adler CE, Miyoshi-Akiyama T, Aleman LM, Tanaka M, Smith JM, Mayer BJ . 2000. Abl family kinases and Cbl cooperate with the Nck adaptor to modulate Xenopus development. J Biol Chem 275:36472-36478.

Lei M, Lu W, Meng W, Parrini MC, Eck MJ, Mayer BJ , Harrison SC. 2000. Structure of PAK1 in an autoinhibited conformation reveals a multi-stage activation switch. Cell 102:387-397.

Mayer, BJ. 2000. Using protein-interaction domains to manipulate signaling pathways. In: Signalling networks and cell-cycle control: The molecular basis of cancer and other diseases, Gukind JS, ed. Totowa, NJ: Humana Press, 439-452.

Kieran MW, Katz S, Vail B, Zon LI and Mayer BJ . 1999. Concentration-dependent positive and negative regulation of a MAP kinase by a MAP kinase kinase. Oncogene 18:6647-6657.

Smith JM, Katz S, and Mayer BJ. 1999. Activation of the Abl tyrosine kinase in vivo by Src Homology 3 domains from the SH2/SH3 adaptor Nck. J Biol Chem 274:27956-27962.

Parrini MC and Mayer BJ. 1999. Engineering temperature-sensitive SH3 domains. Chem Biol 6:679-687.

Lu W and Mayer BJ. 1999. Mechanism of activation of Pak1 kinase by membrane localization. Oncogene 18:797-806.

 
LAB MEMBERS
Bruce Mayer Principal Investigator bmayer@neuron.uchc.edu

Kazuya Machida

Assistant Professor machida@neuron.uchc.edu
Lin Jia Postdoctoral Fellow linjia@uchc.edu
Susumu Antoku Graduate Student antoku@student.uchc.edu
Sofya Borinskaya Graduate Student SBorinskaya@student.uchc.edu
Jonathon Ditlev Graduate Student jditlev@student.uchc.edu
Hao Lu Graduate Student hlu@student.uchc.edu
Ji Zheng Graduate Student jizheng7@yahoo.com
Christopher Thompson Research Assistant cthompson@neuron.uchc.edu
 
ROTATIONS
Several rotation projects are available. For more information contact Bruce Mayer.