Validation and QC Services
The UConn Stem Cell Core offers
validation and quality control (QC) services applicable to
human embryonic stem (ES) cells and human induced
pluripotent stem (iPS) cells. They may be useful for
initial derivation and characterization, prior to
experiments, supplementation to publication submissions, as
well as for general tissue culture lab QC at regular
intervals.
These validation and QC services and
reagent are available from the UConn Stem Cell Core:
Mycoplasma
testing: The UConn Stem Cell Core mycoplasma testing
service is performed using the Myco-Alert kit from Lonza.
Mycoalert kit FAQ
For more
information about mycoplasmal biology and importance, see
Bionique Laboratories excellent
FAQ: (Note: Bionique is a commercial testing service.)
Immunostaining
kit: to detect pluripotency markers including cell
surface markers, e.g., SSEA-3, SSEA-4, TRA1-60, and TRA1-81,
and intracellular markers, e.g., NANOG if no exogenous NANOG
was introduced to the cells for reprogramming. The Stem
Cell Core will provide you with aliquots of the antibodies
that you need, as well as protocols.
Gene
expression by RT-PCR kit: to determine expression of
endogenous OCT4, NANOG, SOX2, LIN28,
KLF4, and c-MYC. The Stem Cell Core will
provide you with aliquots of the primers that you need, as
well as protocols.
Teratoma
formation: to test pluripotency in vivo: cells
are injected into Immunity-deficient mice. Any tumors that
form are analyzed to look for tissues representing all three
germ layers. The Stem Cell Core will perform this service
for you.
To inquire about or obtain services,
please visit the
stem cell core store or contact Jung Park at
park@uchc.edu
The following validation and QC
services are available from outside vendors. Please contact
the vendor for details. Please note, we have listed
services of which we are aware. The list is not
comprehensive.
G-band Karyotyping and DNA profiling:
Cell Line
Genetics:
http://www.clgenetics.com
WiCell Cytogenetics:
http://www.wicell.org/index.php?option=com_content&task=section&id=21&Itemid=267
Adventitious
virus testing:
GlobalStem:
http://www.globalstem.com
Apptec:
http://www.wuxiapptec.com/
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