GLO-Worm is being developed as a database of expression and localization of GFP reporters during the development of the nematode C. elegans. We are using two distinct fluorescence imaging technologies to record the dynamics of expression and localization of GFP within live worms and embryos. Each instrument was chosen specifically to maximize resolution, throughput, and interpretability of data from either the embryonic or postembryonic stages of C. elegans development.:

2-photon microscope: This instrument employs femtosecond-pulsed infrared laser, galvanometer scan control, and non-descanned fluorescence detection to obtain inherently confocal images. It maximizes both long-term viability of the specimen and efficiency in detecting fluorescent photons and is used for high 3D-resolution in multi-hour 4D recordings of live embryos. Both interactive QTVR movies and EDEC chronograms are produced from raw data captured by the 2-photon scope.

BIOSORT Profiler: This instrument employs visible laser excitation and liquid flow of suspended worms through an optical chamber to obtain 1-dimensional traces of fluorescence intensity along the body length. Its flow apparatus allows reliable head-to-tail elongated orientation of live worms, otherwise impossible on microscope stage. Hundreds of animals can be imaged per minute. The traces are assembled into PDEC chronograms.